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Fig. 2 | Cell & Bioscience

Fig. 2

From: Myelin basic protein enhances axonal regeneration from neural progenitor cells

Fig. 2

Mbp necessary for myelin-induced dendrite regeneration in NPCs. a–g Analysis of neuritogenesis in wild-type (WT) or Mbp-knockout (Mbp−/−) spinal cord-derived E12 mouse neural progenitor cells (E12 mNPCs) after 48 h of Poly-d-lysine control (PDL Ctrl) or mouse myelin (Mye) substrate culture. WT PDL Ctrl was used to normalize values for all experiments. a Representative images of axonal βIII-tubulin labeling in E12 mNPCs. Scale bar: 30 μm. b Total length of neurites per cell, c longest neurite per cell, d neurite branching for each cell, and e neurite initiation per cell. f, g Total length of neurites per cell from E12 to E17. Scale bars, 30 μm (f). h–l Analysis of neuritogenesis in WT or Mbp−/− spinal cord-derived E14 rat neural progenitor cells (E14 rNPCs) after 48 h of PDL Ctrl or rat Mye substrate culture. WT PDL Ctrl was used to normalize values for all experiments. h Total length of neurites per cell, i longest neurite per cell, j neurite branching for each cell, and k neurite initiation per cell. l Total length of neurites per cell from E14 to E19. m–r Analysis of neuritogenesis in D1 WT or Mbp−/− NPCs derived from human iPSCs (D1 hNPCs) after 48 h of Lam Ctrl or rat Mye substrate culture. As Lam substrate is required to culture hNPCs, Lam Ctrl was used to normalize values for each experiment. m Representative images of axonal βIII-tubulin labeling in E12 mNPCs. Scale bar: 20 μm. n Total length of neurites per cell, o longest neurite per cell, p neurite branching for each cell, and q neurite initiation per cell. r Total length of neurites per cell from D1 to D6. All panels report means ± standard deviations (SDs). n = 3 embryos/genotype × 3 wells/embryo. *P < 0.05, **P < 0.01 [two-way ANOVA, post-hoc Tukey’s test]

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