Fig. 1

R-based bioinformatics analysis of E12 mNPCs subjected to different substrates. R-based bioinformatics analysis of published RNA-seq data (GEO acc no. GSE98974) from spinal cord-derived E12 mouse neural progenitor cells (E12 mNPCs) that were cultured on Poly-d-lysine control (PDL Ctrl), laminin (Lam), myelin (Mye), or Mye+Lam substrate [false discovery rate (FDR) < 0.1, n = 3 replicates/condition]. a Gene set enrichment analysis (GSEA)-based identification of six discreet gene co-expression modules (M1-6). Red coloring denotes a positive NES score, while blue coloring denotes a negative NES score. b The network plot and associated hub genes for the M6 gene co-expression module. c Heatmap of differentially-expressed genes (DEGs) ordered by descending log2 fold-change. Upregulation is denoted by green coloring, while downregulation is denoted by red coloring. e Venn diagram visualizing overlapping upregulated DEGs associated with Mye substrate. f qPCR of Mbp mRNA expression and g immunoblotting of Mbp in whole-cell lysates from E12 mNPCs on PDL Ctrl or myelin (Mye) substrates. Gapdh is the housekeeping and loading control. h qPCR of Mbp mRNA expression and i immunoblotting of Mbp protein expression in mNPCs reveals maturation-based Mbp downregulation on Mye substrate. All panels report means ± standard deviations (SDs). n = 3 embryos/genotype × 3 wells/embryo. *P < 0.05, **P < 0.01 [e, f Student’s t-test; g, h one-way ANOVA, post-hoc Tukey’s test]