Fig. 6

PTEN mediated electrotaxis via maintenance of biased pseudopod protrusion toward the cathodal forwarding direction. PHCrac-GFP containing pseudopod protrusions were quantified. a-b EF triggered a biased pseudopod redistribution at the anterior of electrotaxing WT cells. Two representative consecutive time points are shown. Time interval = 20 s. c and g Distribution of total pseudopods number of 12 EF-treated WT or pten⁻ cells, respectively. d and h Distribution of total time spent in each category from pseudopods of 12 EF-treated WT or pten⁻ cells, respectively. e and f EF-triggered pseudopod anterior redistribution was fully abolished in pten⁻cells. Two representative consecutive time points are shown. Time interval = 20 s (i) Comparing the average new pseudopods number generated between a single WT and pten⁻ cell, in the absence or presence of EF for 320 s. j Comparing total pseudopods time generated between a single WT and pten⁻ cell, in the absence or presence of EF for 320 s. k Comparison studies of pseudopod number and time in orientation of 12 EF-treated WT or pten⁻ cells, respectively. Recording time = 320 s. All data are confirmed from minimal 3 independent experiments