Fig. 3

Myosin II redistributed to the posterior plasma membrane of electrotaxing cells, which is depended on actin polymerization and PTEN signaling. a–e Time-lapse images showing translocation and asymmetric redistribution of Myosin II to the posterior plasma membrane of the WT cells in EF (10 V/cm). f, g Latrunculin A treatment abolished asymmetric membrane translocation of myosin-GFP in EF. EF = 30 V/cm. Similar results were observed in 34 cells from a minimum of three independent experiments. h–k Washout of latrunculin A restored the asymmetrical redistribution of myosin GFP to the posterior plasma membrane of the electrotaxing WT cells. See Movie “Smovie11_myosinGFP_EF_LatA_washout”. EF = 30 V/cm. The same results were observed in 53 cells from 3 independent experiments. l-q Myosin-GFP/pten⁻ lost plasma membrane redistribution of myosin II in EF (30 V/cm). e, g, k, m, o, q fluorescence intensity line-scan of myosin-GFP along the lines indicated in d, f, j, l, n and p, respectively. r GFP intensities at the plasma membrane were determined relative to that in the cytosol. Values represent the mean ± s.d. (n⩾15)