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Fig. 1 | Cell & Bioscience

Fig. 1

From: Electric signals counterbalanced posterior vs anterior PTEN signaling in directed migration of Dictyostelium

Fig. 1

pten null mutation significantly reduced electrotaxis at lower voltages, which was recovered by re-expression of pten. a-c Images selected from time-lapseVideos showing the migrating cells. Red lines and blue arrows are cell trajectories and endpoints over 15 min. d-f Composite trajectories of migrating cells with the starting points placed at the origin. b and e pten cells showed significantly reduced electrotaxis. c and f The re-expression of wild type pten on pten (PTEN/pten, or pten rescue) completely reversed the electrotaxis defects in the pten cells. g, h voltage dependence migration directedness and trajectory speed. *, P < 0.05; **, P < 0.01, compared with no EF control; #, P < 0.05; ##, P < 0.01 compared between WT/pten rescue groups and pten group, one-way ANOVA. A minimal 150 cells were analyzed from each experimental group. A minimal 3 repeats were conducted for all conditions investigated. EF = 10 V/cm in a–f, with cathode to the right

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