Fig. 5

The association of EIF5A2 with the TGFβ pathway and inhibition of EIF5A2 attenuated TGFβ signaling pathway in ovarian cancer cells. a TGFβ induced EIF5A2 expression in SKOV3 and OVCA8 cells at the indicated time points as detected by Western blot. b TGFβR1/2 inhibitor SB431542 inhibited EIF5A2 expression in SKOV3 and OVCAR8 cells following 24 h treatment as detected by Western blot. c Western blot analysis of EIF5A2 and TGFβRII in TGFβR2 KD and control SKOV3 and OVCAR8 cells following 6 ng/ml TGFβ treatment for 24 h, respectively. d, e Western blot analysis of phospho- and total SMAD2 in EIF5A2 KO and control SKOV3 and OVCAR8 cells or in EIF5A2 expressing and control OVCAR3 following 6 ng/ml TGFβ treatment at the indicated time points. (*p < 0.05; **P < 0.01; ***P < 0.001)