Fig. 5
From: Aldose reductase deficiency inhibits LPS-induced M1 response in macrophages by activating autophagy
AR deficiency facilitates IKKβ and IKKγ to co-localize with autophagosomes and lysosomes in LPS-stimulated macrophages. Confocal microscopy of BMMs from WT or AR KO mice treated with or without 500 ng/ml LPS 1 h and co-stained for a beclin1 and IKKβ, b beclin1 and IKKγ, c LC3 and IKKβ, d LC3 and IKKγ, e LAMP1 and IKKβ, and f LAMP1 and IKKγ. Scale bars = 20 µm. Right graphs represent the quantification of co-localization of signals using Manders’ overlap coefficient. Data are expressed as the mean ± SD from three independent experiments, *P < 0.05, **P < 0.01