Fig. 2

Stimulation of AR-deficient macrophages with LPS decreases the protein levels of IKKβ and IKKγ. a BMMs from WT or AR KO mice were treated with 500 ng/ml LPS for the indicated time periods, and the production of iNOS was assayed. b Effect of AR deficiency on several important components of the TLR4/NF-κB signaling pathway. Immunoblot analysis for TLR4, p-IKKα/β, IKKα, IKKβ, IKKγ, IκBα, p-IκBα, p65, p-p65, and iNOS in BMMs from WT or AR KO mice treated with or without 500 ng/ml LPS for 16 h. c qRT-PCR of IKKβ, IKKγ, iNOS, and CD86 mRNA in BMMs from WT or AR KO mice treated with or without 500 ng/ml LPS for 16 h. Transcription levels are expressed as fold change of control. d BMMs from WT or AR KO mice were treated with 500 ng/ml LPS for the indicated time periods. Data represent the mean ± SD of three different experiments