Fig. 6

LPS-mediated induction of HECTD1 involves translocation of FOXJ2 into the nucleus. a Illustration of the consensus binding site of FOXJ2 to the HECTD1 promoter. b ChIP assay demonstrated LPS-mediated binding of FOXJ2 to the HECTD1 promoter. c-e Time-dependent effect of LPS on the total level of FOXJ2 in whole cells (c), nucleus (d), and cytosol (e). *p < 0.05 and ***p < 0.001 vs. the control group. f Pretreatment of primary mouse astrocytes with BD1047, SB203580, and SP600125 significantly inhibited LPS-mediated translocation of FOXJ2 into the nucleus. All the data are presented as mean ± SEM of three individual experiments. **p < 0.01 vs. the control group; ##p < 0.01 and ###p < 0.001 vs. the LPS-treated control group. g Pretreatment of primary mouse astrocytes with siRNA of σ-1R, JNK, or p38 significantly inhibited LPS-mediated translocation of FOXJ2 into the nucleus. All the data are presented as mean ± SEM of three individual experiments. **p < 0.01 vs. the control group; #p < 0.05 and ##p < 0.01 vs. the LPS-treated control group