Fig. 2
From: Involvement of HECTD1 in LPS-induced astrocyte activation via σ-1R-JNK/p38-FOXJ2 axis
Role of HECTD1 in astrocyte activation induced by LPS in vitro. a, b Representative immunoblots and the densitometric analysis from three separate experiments showed the effect of HECTD1 NIC and ACT transfection with/without 100 ng/ml LPS treatment on GFAP and HECTD1 expression. Transfection with HECTD1 NIC inhibited LPS-induced GFAP expression (a). Transfection with HECTD1 ACT enhanced LPS-induced GFAP expression (b). c, d Representative images of HECTD1 fluorescence (green) and GFAP fluorescence (red) showed the effect of HECTD1 NIC and ACT transfection with/without 100 ng/ml LPS treatment on GFAP and HECTD1 expression. Scale bar = 50 μm. Quantification of GFAP and HECTD1 immunofluorescence intensity used Image J software. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. the control group; #p < 0.05, ##p < 0.01, and ###p < 0.001 vs. the LPS-treated control group