Fig. 5

Increased intracellular damages after AUY-VE. A673 cells were treated with 45 nM AUY922 ± 2 µM VE821 for 24 h. a Intracellular structures were analyzed by transmission electron microscopy (TEM). Red arrows indicate lipid-filled vesicles; yellow arrows indicate lysosomes. b Golgi networks were analyzed by fluorescence microscopy of GM130. Red arrows indicate dispersed Golgi structures. c Endoplasmic reticulum (ER) networks were analyzed by fluorescence microscopy of GRP94. Red arrows indicate vesicular ER structures. A673 cells were treated with 1–2 µg/ml of tunicamycin for 24 h. e ER networks were analyzed by fluorescence microscopy of GRP94. c–e DAPI was used to stain cell nuclei. A shows one representative experiments; B-E are representative for at least two independent experiments