Fig. 2

Decreased cell proliferation after AUY-VE in WE-68 cells. WE-68 (a) and A673 (b) cells were treated with 15–45 nM of AUY922, 2 µM of VE821, 5 µM of KU55933 and their combinations. Cell densities at 24, 48 and 72 h after treatment were assessed by crystal violet-based cell proliferation assay. DMSO was used as control. c Representative images of a BALB/c cell transformation assay (BALB-CTA) after 72 h of treatment with 15–45 nM AUY922 ± 2 µM VE821 or 5 µM KU55933. MCA/TPA-transformed cell foci are Giemsa stained and appear in blue. Quantification of the number of type-III foci/well from BALB-CTA experiments is shown in D. All graphs show the mean ± SEM of three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001)