Fig. 1
From: Cooperative treatment effectiveness of ATR and HSP90 inhibition in Ewing’s sarcoma cells
Increased apoptosis after ATRi and HSP90i combinations. p53 wild-type WE-68 and p53 null A673 cells were treated with the indicated concentrations of AUY922, VE821, KU55933 and their combinations. DMSO was used as control. a–d The loss of the mitochondrial transmembrane potential (ΔΨM) and cell death were assessed by flow cytometry after 48 h. All graphs show the mean ± SEM of three independent experiments. Statistical analysis was done using two-way ANOVA test (*p < 0.05; **p < 0.01; ***p < 0.001). WE-68 (e) and A673 (f) cells were treated with 15–45 nM of AUY922, 2 µM of VE821, 5 µM of KU55933 and their combinations. Analysis of indicated proteins was done by Western blot after 24 h; vinculin was used to control protein loading. Immunoblots are representative of at least two independent experiments. Quantification of selected proteins is shown in g