Fig. 5

Selinexor attenuates fly developmental lethality and reduction in tracheal branching induced by SARS-CoV-2 Orf6 transgene expression. a Graphic presentation of conservation SARS-CoV-2 Orf6 interaction with human host proteins. Graph summarizes the high-confidence interactions between SARS-CoV-2 Orf6 protein and human proteins described in Gordon et al. [10] and our study [22]. Human interacting proteins are colored based on their conservation score (i.e. DIOPT score [13]) of the best matching fly ortholog. Numeric conservation scores human-fly proteins have been provided in the table. b Quantification of mortality rate prior to eclosion for wild type (w¹¹¹⁸) or SARS-CoV-2 Orf6, Nsp6 or Orf7a transgenic flies [overexpression (OE) driven by ubiquitous enhancer Tubulin (Tub)] following treatment with different doses of Selinexor. Mortality calculated as: (long hair − short hair) / short hair × 100. Four replicates, each replicate N = 50 flies per group. c Representative images of tracheal branches (taken at location equivalent to red box in Fig. 3b) in wild type (w¹¹¹⁸) and SARS-CoV-2 Orf6 overexpression (OE) transgenic flies, with (0.2 μM) or without (0 μM) Selinexor treatment. Arrow indicates a missing class II terminal branch based on typical wild type pattern. d Quantification of tracheal branch numbers in one segment for observations in (c). N = 6 larvae per group. Results are presented as mean ± SD. Statistical significance (*) is defined as P < 0.05