Fig. 5

A duplication event of wild-type DNA during homologous recombination of the L1L2_Bact_P cassette (see Fig. 1a for details) into the Agmo locus. a Schematic drawing of the homologous recombination event of the transgenic cassette into the wild-type Agmo locus (indicated by the dashed grey line) and the tandem duplication event of a 94 kb Agmo wild-type segment inserting unmodified exons 1–3 of the Agmo locus upstream of the cassette (indicated by the dashed red lines). The numbers refer to chromosome 12 of the mouse, assembly GRCm38.p6 accession NC_000078.6. A potential mechanism leading to this duplication event is outlined inDiscussion section. b PCR across the duplication breakpoint (see Additional file 1: Fig. S10) in homozygous Agmo-lacZ animals (tg/tg), stem cells used for generation of the Agmo-lacZ mouse and in wild-type mice (wt/wt)