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Fig. 1 | Cell & Bioscience

Fig. 1

From: When the genome bluffs: a tandem duplication event during generation of a novel Agmo knockout mouse model fools routine genotyping

Fig. 1

Agmo knockout-first allele construct and confirmation of cassette integration in the Agmo region of the mouse genome. a EUCOMM construct for Agmo knockout-first (Agmo-lacZ) allele. The critical exon 2 is flanked by loxP sites to create a floxed allele for conditional knockout. pA: polyadenylation sequence; FRT: FLP recognition target. b, c FISH experiments in heterozygous Agmo knockout (Agmo-lacZ tg/wt, b) and wild-type (WT, c) fibroblasts using BAC probes against chromosome 12 (red) and the cassette (green, red arrow). Insets show a 2x magnification of the region of interest. Fiber-FISH experiments show integration of the cassette (red signal, indicated by the red arrow) in the middle of the Agmo locus (green signal) in heterozygous cells (d). Wild-type fibers (e) showed no signal for the cassette

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Cell & Bioscience

ISSN: 2045-3701

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