Fig. 6

Inhibition of GRP75 alleviates the apoE4 (Δ272–299)-induced mitochondrial impairment in N2a cells. N2a cells were transiently transfected with the plasmid pIRES or apoE4 (Δ272–299), and treated with, or without, 1 µM GRP75 inhibitor MKT077 for 18 h. Furthermore, the apoE4 (Δ272–299)-transfected N2a cells were transfected with 50 nM control or GRP75-specific siRNA for 18 h. a, g Western blot analysis of the relative levels of GRP75 expression in N2a cells. b, c, h, i Western blot analysis of the relative levels of MFF, MFN2, MFN1 and OPA1 expression and DRP1 phosphorylation in N2a cells. d, j The MMP was analyzed by the JC-1. e, k The levels of ATP were analyzed by chemiluminescence. f, l The levels of ROS were analyzed by DCFH-DA. m Longitudinal analysis of Ca²⁺ levels in mitochondria of N2a cells. Data are representative images or expressed as the mean ± SEM of each group (n = 3) from three separate experiments. **P < 0.01, *P < 0.05 versus the control N2a cells; ^##P < 0.01, ^#P < 0.05 versus the apoE4 (Δ272–299)-expressed N2a cells. The significant differences were determined by ANOVA and post hoc Fisher’s least significant difference