Fig. 5

In vivo efficacy evaluation of EGFL6 antibody. a 16 six-week-old nude mice were injected subcutaneously with the same volume of Matrigel, and 1\(\times\)10⁷ of HCT-116 cells into the right flank of each animal. The tumor volume and body weight observation in HCT-116 xenograft model treated with two groups: control (IgG, i.p, twice/week, n = 8) and EGFL6-E5-IgG (10 mg/kg, iv, twice/week, n = 8). b Eight-week-old balb/c mice (NLAC, Taipei, Taiwan) were injected with CT-26 cells (2\(\times\)10⁵ cell/mouse) intravenously and then divided into three groups for treatments: control (IgG, 20 mg/kg, n = 8), EGFL6-E5-IgG (20 mg/kg, n = 6) and bevacizumab (20 mg/kg, n = 6). Antibodies were given every 5 days by tail vein injection. Body weight was monitored every 2 or 3 days. Scale bar represents 1 cm. c A total of 11 seven-week-old nude mice (NLAC, Taipei, Taiwan) were used in wound healing model, 3 groups of mice treated with Control IgG (20 mg/kg, n = 5), EGFL6-E5-IgG (20 mg/kg, n = 5), and bevacizumab (20 mg/kg, n = 5). Treatments were given intravenously for a consecutive five-on-two-off regimen. Observed for 1 week until Control healed. d A total of twelve nude mice (NLAC, Taipei, Taiwan) were divided into four groups. For basal group (n = 2), mice were injected with 500 µl matrigel subcutaneously. For control group (n = 5), mice were injected with 500 µl matrigel subcutaneously mixed with endothelial growth factor (EGF, 150 ng/mL) and heparin (10 µl). For indicated antibody group (n = 5), mice were injected with 500 µl matrigel subcutaneously mixed with endothelial growth factor (EGF, 150 ng/mL), heparin (10 µl), and treated anti-EGFL6 antibody (15 mg/kg, iv, q5D). *p < 0.05, **p < 0.01