Fig. 3

EGFL6 regulates CRC cell migration and invasion. a, b Colony formation assay to test the proliferation of HCT116 and HT29 after silencing EGFL6. c, d The migration and invasion assay of HCT116 cell after silencing EGFL6. Scale bar represents 100 µm. e The mRNA level of EGFL6 and ADAMTS1 (a disintegrin and metalloprotease with thrombospondin motif 1) under normoxia and hypoxic conditions to validate the signaling relationship between EGFL6 and HIF-1α. N = normoia, H = hypoxia. f HIF-1α and EGFL6 expression under hypoxia condition. N = normoia, H = hypoxia. g The mRNA expression of invasion and migration-associated MMP (matrix metalloproteinase)-2, MMP-9, ADAMTS1 and Snail after silencing EGFL6. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001