Fig. 1

EGFL6 expresses in CRC patients and in early-developed colon carcinogenesis animal model. a EGFL6 (Epidermal growth factor-like protein 6) expression in human CRC (colorectal cancer) tissue with indicated stages under 100X magnification. EGFL6 staining score was “++” for stage I to IV. b EGFL6 staining scoring of non-tumor part and tumor part were showed. Normal group (n = 5) and human CRC group stage I (n = 6), stage II (n = 10), stage III (n = 9), stage IV (n = 9). c AOM (Azoxymethane) mouse model schedule. Total number of 35 eight-week-old A/J mice were randomly divided into control or AOM treatment group. Mice were sacrificed and colons were collected at 1, 2, 4, 8, and 18 weeks after the last AOM challenge to assess for methylene blue, H&E (hematoxylin and eosin stain) staining, and IHC (immunohistochemistry) staining of EGFL6. d Methylene blue staining of intestinal tissue (from anus to the cecum) to observe changes in the intestines. Stained with 0.05% methylene blue. e IHC staining of intestinal tissues in different time points after AOM stimulation. Scale bar represents 50 µm. **** p < 0.0001