The YAP/HIF-1α/miR-182/EGR2 axis is implicated in asthma severity through the control of Th17 cell differentiation

Table 2 Primer sequences for reverse transcription quantitative polymerase chain reaction

Primer	Sequence (5′–3′)
hYAP	F: TAGCCCTGCGTAGCCAGTTA R: TCATGCTTAGTCCACTGTCTGT
mYAP	F: CCCTCACCCTCCCTGAAATCTACAA
mYAP	R: AGCATTCCACAGGTCCAAGGCAGA
hHIF-1α mHIF-1α hmiR-182 mmiR-182 hEGR2 mEGR2 hRORγt mRORγt hGAPDH mGAPDH hU6 mU6	F: GCTGGCCCCAGCCGCTGGAG R: GAGTGCAGGGTCAGCACTAC F: AGCTTCTGTTATGAGGCTCACC R: TGACTTGATGTTCATCGTCCTC F: ACACTCCAGCTGGGTTTGGCAATGGTAGAACT R: TGGTGTCGTGGAGTCG F: TGCGGTTTGGCAATGGTAGAAC R: TGGTGTCGTGGAGTCG F: CTTTGACCAGATGAACGGAG R: CCCATGTAAGTGAAGGTCTG F: CTTCAGCCGAAGTGACCACC R: GCTCTTCCGTTCCTTCTGCC F: GCTGTGATCTTGCCCAGAACC R: TGCCCATCATCATTGCTGTTAATCC F: TGCAAGACTCATCGACAAGG R: AGGGGATTCAACATCAGTGC F: GGAGCGAGATCCCTCCAAAAT R: GGCTGTTGTCATACTTCTCAGG F: AGGTCGGTGTGAACGGATTTG R: TGTAGACCATGTAGTTGAGGTCA F: GTAATACGACTCACTATAGGGAGAAGAG R: CGCGCCTGCAGGTCGAC F: GCTTCGGCAGCACATATACTAAAAT R: CGCTTCACGAATTTGCGTGTCAT
hmiR-183	F: GAGGATCCCCGGGTACCAAGGGAGTGGGCAGGCTA R: ATAAGCTTGATATCGTCCCTGCACCCTTGGAAGCA
mmiR-183	F: CGCGCTAT GGCACTGGTAG
mmiR-183	R; GTGCAGGGTCC GAGGT
hmiR-96	F: TTC TTC TAG AGG AAG CCC TAA TCC
hmiR-96	R: TCC CTT CTA GAA AGA TCT ACT CCC C
mmiR-96	F: GCCC GCTTTGGCACTAGCACATT R: CAGTGCAGGGTCC GAGGT
hEGR2	F: CAGGTGTCCACTCCCAGGTCCAAG R: GGCAACTAGAAGGCACAGTCGAGG

YAP Yes-associated protein, HIF-1α hypoxia-inducible factor 1α, miR microRNA, EGR2 early growth response 2, F forward, R reverse, GAPDH glyceraldehyde-3-phosphate dehydrogenase, h before gene name, Homo sapiens (human); m before gene name, Mus musculus (house mouse)

ISSN: 2045-3701