Fig. 5

miR-182 inhibits EGR2 expression to promote Th17 cell differentiation. a the binding site between miR-182 and EGR2 3′UTR in mice predicted using starbase; b miR-182 expression determined by RT-qPCR and the binding of miR-182 to EGR2 confirmed by dual luciferase reporter gene assay; c miR-182 expression and EGR2 mRNA expression determined by RT-qPCR; d Western blot analysis of the EGR2 protein in cells after different treatments; e the Th17 cell proportion after different treatments determined by flow cytometry; f the level of IL-17A after different treatments by ELISA; g RORγt mRNA expression in cells after different treatments determined by RT-qPCR; h Western blot analysis of the RORγt protein in cells after different treatments. Comparisons between two groups were conducted by unpaired t test, and those between multiple groups were conducted by one-way ANOVA with Tukey’s post hoc test; the experiments were repeated 3 times independently; * p < 0.05, compared with cells treated with mimic-NC, inhibitor-NC, oe-NC + mimic-NC, or sh-NC + inhibitor-NC; # p < 0.05, compared with the cells treated with miR-182 mimic + oe-NC or miR-182 inhibitor + sh-NC