Fig. 2

YAP and HIF-1α promote the differentiation of CD4⁺ T cells into Th17 cells. a mRNA expression of YAP and HIF-1α in Th17 cells determined by RT-qPCR; b Western blot analysis of YAP and HIF-1α proteins in Th17 cells; c Western blot analysis of YAP and HIF-1α proteins in Th17 cells following different treatments; d Th17 cell proportion after different treatments detected by flow cytometry; e IL-17A serum level in cell supernatant after different treatments by ELISA; f RORγt mRNA expression in cells after different treatments determined by RT-qPCR; g Western blot analysis of RORγt protein in cells after different treatments. Comparisons between two groups were conducted by unpaired t test, and those among multiple groups were conducted by one-way ANOVA with Tukey’s post hoc test; the experiments were repeated 3 times independently; * p < 0.05, compared with the CD4⁺ T cells, or cells treated with sh-NC, oe-NC, or sh-NC + oe-NC; # p < 0.05, compared with the cells treated with oe-YAP + sh-NC