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Fig. 6 | Cell & Bioscience

Fig. 6

From: Deletion at an 1q24 locus reveals a critical role of long noncoding RNA DNM3OS in skeletal development

Fig. 6

LncRNA- Dnm3os sustains the proliferation of chondrocytes by upregulating NGF. a EdU labeling of proliferating Atdc5 cells and b calculation thereof. The ATDC5 cells transfected by RK5, FL, DKO, and miR214 were treated with vehicle of PD90780 (1‰ DMSO) or PD90780 (10ug/1ml) for 24 hours, and one day later the cells were labeled with EdU for 8 h before visualization. The frequency of nuclear EdU labeling is determined by examination of at least three random fields, magnification ×400 and at least 300 cells and nuclei in each group. c Alcian blue staining and d quantification thereof of cartilage matrix produced by the differentiated ATDC5 cells. The ATDC5 cells were transfected and treated with PD90780 as in (a). eh RT-qPCR quantifications of Col1a1, Col2a1, Mmp9 and Col10a1 in ATDC5 cells transfected and treated with PD90780 as in (A). Student T-test was used for statistical analysis. *P < 0.1, **P < 0.01, ***P < 0.001, and ns, not significant

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