Fig. 4

LncRNA-Dnm3os is required for maintaining the proliferative potential of the ATDC5 cells. a EdU labeling of proliferating ATDC5 cells and b calculation thereof. The ATDC5 cells were transfected by RK5, FL and DKO individually, and one day later the cells were labeled with EdU for 8 hours before visualization. c Alcian blue staining of cartilage matrix produced by differentiated ATDC5 cells. d EdU labeling of proliferating ATDC5 cells and e calculation thereof. The ATDC5 cells were transfected with scrambled shRNA, shDnm3os-1 and shDnm3os-2 individually, and one day later the cells were labeled with EdU for 8 h before visualization. The frequency of nuclear EdU labeling in (a) and (d) were determined by examination of at least three random fields, magnification ×400 and at least 300 cells and nuclei in each group. One-way ANOVA test was used for statistical analysis. ***P < 0.001, ****P < 0.0001, and ns, not significant