Fig. 3

LncRNA-Dnm3os sustains the proliferation of chondrocytes independent of the co-cistronic microRNAs. a Alcian blue staining and b quantification thereof of cartilage matrix produced by differentiated chondrocytes. c Alkaline phosphatase staining and d quantification thereof of differentiated chondrocytes. The primary articular chondrocytes were transfected with the control vector or constructs that express FL or the mutant Dnm3os prior to induction of chondrogenic differentiation. e EdU labeling of proliferating chondrocytes and f calculation thereof. The primary articular chondrocytes were transfected as in (a–c), and one day later the cells were labeled with EdU for 8 hours before visualization. The frequency of nuclear EdU labeling is determined by examination of at least three random fields, magnification ×400 and at least 300 cells and nuclei in each group. One-way ANOVA test was used for statistical analysis. **P < 0.01, ***P < 0.001