Fig. 8
From: Identification of a potent antagonist of smoothened in hedgehog signaling
0025A inhibits the expression of Gli1 induced by SAG. a Serum-starved NIH3T3 cells were induced with DMSO or SAG for 24 h. Cells were analyzed for mRNA levels of Gli1. b, c Serum-starved NIH3T3 cells were induced by 10–7 M (100 nM) SAG with vehicle, different concentration of 0025A, or 1 μM GDC-0449 (GDC-1) for 24 h. Cells were analyzed for mRNA levels (b) and the protein levels (c) of Gli1. d Quantitation of Gli1 protein levels normalized to Tubulin loading control. All data are means ± SEM (student’s t-test). **P < 0.01, ***P < 0.001