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Fig. 7 | Cell & Bioscience

Fig. 7

From: Identification of a potent antagonist of smoothened in hedgehog signaling

Fig. 7

0025A inhibits the expression of Gli1 induced by Shh-CM. a Serum-starved NIH3T3 cells were treated with Shh-CM for 24 h. Cells were analyzed for mRNA levels of Gli1. b-c Serum-starved NIH3T3 cells were treated by Shh-CM with vehicle, different concentration of 0025A, or 1 μM GDC-0449 (GDC-1) for 24 h. Cells were analyzed for mRNA levels (b) and the protein levels (c) of Gli1. d Quantitation of Gli1 protein levels normalized to Tubulin loading control. All data are means ± SEM (student’s t-test). **P < 0.01, ***P < 0.001

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