Fig. 5
From: Identification of a potent antagonist of smoothened in hedgehog signaling
0025A competitively replaces Bodipy-cyclopamine binding to wild-type Smo and mutant Smo-D473H. a Competitive binding of Bodipy-cyclopamine to wild-type Smo with different concentration of Smo antagonists: 0025A, GDC-0449 (GDC), cyclopamine (Cyc). The Bodipy-cyclopamine competitive assay was performed in HEK293 cells transiently transfected with human wild-type Smo. b Competitive binding of Bodipy-cyclopamine to mutant Smo with 10–6 M (1 μM) Smo antagonists. The Bodipy-cyclopamine competitive assay was performed in HEK293 cells transiently transfected with human mutant Smo-D473H. The results of Bodipy-cyclopamine binding (green) were analyzed by flow-cytometry. All data are means ± SEM (student’s t-test). **P < 0.01, ***P < 0.001