Fig. 1
From: Identification of a potent antagonist of smoothened in hedgehog signaling
The translocation of βarr2-GFP to Smo in U2OS cells. The U2OS cells stably overexpressing βarr2-GFP alone (a) or βarr2-GFP and Smo-633 (b-d). Cells were treated with DMSO vehicle (b), 1 μM cyclopamine (Cyc) (c), or 1 μM Cyc and 1 μM SAG (d) for 24 h at 37 ℃. The arrows showed the intravesicular aggregation of βarr2-GFP. e Percentage of the number of βarr2-GFP aggregation was quantitated. Scale bar, 10 µm