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Fig. 4 | Cell & Bioscience

Fig. 4

From: Long non‐coding RNA NEAT1 transported by extracellular vesicles contributes to breast cancer development by sponging microRNA-141-3p and regulating KLF12

Fig. 4

High NEAT1 expression in EVs promotes chemotherapy resistance in BC cells. a MTT assay was applied to measure MCF-7 cell proliferation; Cisplatin, paclitaxel and 5-fluorouracil were used to treat the cells respectively. The cell activity decreased with the increase of drug concentration. EVs weakened the drug effect and increased cell activity; b colony formation assay was utilized to detect MCF-7 cell colony formation ability; EVs increased the colony formation of MCF-7 cells under the action of drugs; c flow cytometry was utilized to determine MCF-7 cell apoptosis; EVs decreased the MCF-7 cell apoptosis under the action of drugs; d RT-qPCR was applied to detect MDR1 expression in MCF-7 cells; EVs increased MDR1 expression in MCF-7 cells under the action of drugs. Three independent experiments were performed; data were analyzed using two-way ANOVA, and Tukey’s multiple comparisons test was used for pairwise comparisons after ANOVA; * compared with the control group, p < 0.05

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