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Fig. 4 | Cell & Bioscience

Fig. 4

From: DNA primase subunit 1 deteriorated progression of hepatocellular carcinoma by activating AKT/mTOR signaling and UBE2C-mediated P53 ubiquitination

Fig. 4

PRIM1 facilitated aggressive phenotypes and EMT of HCC cells. a The protein and mRNA expression of PRIM1 in HCC cell lines were detected by western blotting and RT-qPCR, respectively. b The colony formation of PRIM1-silenced MHCC97H and PRIM1-overexpressed HepG2. c Following transfection of Kd-PRIM1 or OE-PRIM1 plasmid, the proliferation of HCC cells was detected by CCK-8 assay. d The sorafenib sensitivity was detected in PRIM1-silenced MHCC97H or PRIM1-overexpressed HepG2 cells compared with NC group. e, f Following transfection of Kd-PRIM1 or OE-PRIM1 plasmid, the cell cycle and apoptosis were detected by flowcytometry. g The migration and invasion of MHCC97H or HepG2 cells in each group were detected by transwell assay. h The expression of EMT markers were examined by western blotting with PRIM1 overexpression or downregulation. **P < 0.01; *P < 0.05

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