Fig. 4

Evaluation of particles of the purified samples. a Size distribution of pellets via ultracentrifugation (n = 3). b Size distribution of pellets via SEC (n = 3). c Size distribution of pellets via IAC (n = 3). c Size distribution of pellets via DGUC (n = 3). e The numbers of total particles and sEVs (30–150 nm) in the pellets (n = 3). fRR of the particle and sEVs. g TEM images of the purified samples. e Dyed-gel. Compared of the concentrates, the purified samples showed lower protein content. * P < 0.05