Fig. 2

Distribution of RNA splicing events with significant differences in ESCC after re-annotation with Ensembl and RefSeq gene annotations. a Plot in the left panel indicates exon models of the types of AS assessed by MISO analysis. In the right panel, the different types of AS events before and after re-annotation were quantified with transcript annotations from Ensembl and RefSeq database, respectively. b A total of 283 and 235 splicing events with significantly altered PSI values were identified using transcript annotations from the RefSeq and Ensembl databases, respectively. In total, 169 splicing events were identified in both database transcript annotations. c Percentage of protein coding transcripts (blue) and non-coding transcripts (yellow) representing AS events after re-annotation analysis