Fig. 1

Distribution of RNA splicing events in ESCC after MISO analysis. a Percentages of differentially-spliced AS events with significance in total AS events found in ESCC clinical samples compared to the matched normal samples. b Plot depicts the fraction of events detected for each AS type (blue) compared to events with significantly different expression in ESCC tissues compared to matched normal tissues (red). c Scatter plot of all the AS events identified using MISO. The X-axis represents ΔΨ values, and the Y-axis represents log2 (BF) values. The shape of the dots indicates the type of AS event. Specifically, a hollow square indicates an SE event; cross indicates an RI event; solid square indicates an MXE event; triangle indicates an A5SS event; and circle indicates an A3SS event. Alternative splicing events with BF > 5 and ΔΨ > 0.2 are colored in blue. d Relative fraction of each AS event affected positively or negatively by ESCC. e Percentage of alternative splicing events showing significant changes in SHEE/SHEEC cell line association compared to detectable alternative splicing events showing no difference. f Plot depicts the fraction of events detected for each AS type (blue) compared to events with significantly different expressions in SHEE/SHEEC cell lines (red)