Fig. 3

The immune cells are differentially regulated in spleen in CAE-induced AP. Single cell suspension was prepared from the spleen collected from the same sets of the mice as described in Fig. 1. Multi-dimensional flow cytometry was performed using two mouse immune phenotype Panels as described in Table 1, and data was analyzed by manual gating. Results of the cell counts from three separate experiments were normalized against each of the baseline controls, combined and presented as folds of CON. a Innate immune cells. b B cells. c T cells. d Representative t-SNE showing innate immune cells and B cells measured in Panel 1 on CON and 16 h. e Representative t-SNE showing T cells measured in Panel 2 on CON and 16 h. *p < 0.05 compared with CON