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Fig. 1 | Cell & Bioscience

Fig. 1

From: Characteristic pancreatic and splenic immune cell infiltration patterns in mouse acute pancreatitis

Fig. 1

Innate immune cells comprise the majority of infiltrating inflammatory cells in CAE-induced AP pancreas. AP was induced in adult male C57BL/6J mice by CAE injections (50 µg/kg, 9 injections at hourly intervals, ip). Mice were euthanized at 16 h, 30 h, and day 7 after the last CAE injection (n = 6 mice/group). Untreated mice were used as baseline controls (CON. n = 9 mice). The blood and pancreas were harvested. Single cell suspension was prepared from the pancreas. Multi-dimensional flow cytometry was performed using two mouse immune phenotype Panels as described in Table 1, and data was analyzed by manual gating. Results of the cell counts from three separate experiments were normalized against each of the baseline controls, combined and presented as folds of CON. a Serum amylase levels. b Histopathological scores. c Innate immune cells. d B cells. e T cells. f Representative t-SNE showing innate immune cells and B cells measured in Panel 1 on CON and 16 h. g Representative t-SNE showing T cells measured in Panel 2 on CON and 16 h. *p < 0.05 compared with CON

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