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Fig. 5 | Cell & Bioscience

Fig. 5

From: Neuregulin 1/ErbB4 signaling contributes to the anti-epileptic effects of the ketogenic diet

Fig. 5

KD treatment elevates Nrg1 expression in the hippocampus. a Western blots detecting synaptic proteins in the hippocampus of mice fed with CD or KD, GAPDH serves as the loading control. Shown are representative blots. b Quantitative analysis of data in (a). n = 4 mice per group. Student’s t test, for PSD95, t(6) = 0.5382, P = 0.6098; for Gephrin, t(6) = 0.4457, P = 0.6714; for Synaptotagmin-1, t(6) = 0.03585, P = 0.9726; for GABARa1, t(6) = 0.2533, P = 0.8085; for GluN2B, t(6) = 0.655, P = 0.5367; for GluN2A, t(6) = 0.1.909, P = 0.1048; for GluR1, t(6) = 0.4986, P = 0.6358. c A volcano plot of genes altered by KD treatment in the hippocampus. Genes up-regulated with more than 1.5 fold change with a p-value < 0.05 are depicted in red dots, and those down-regulated with identical fold change and p-value are in green dots. d Quantification of several genes that were reported critical for GABAergic signaling from RNAseq data. n = 3 mice per group. Student’s t test, for Nlgn2 (Neuroligin 2), t(4) = 2.259, P = 0.0868; for Ulk4 2, t(4) = 1.653, P = 0.1737; for Mecp2, t(4) = 2.99, P = 0.083; for BDNF, t(4) = 3.378, P = 0.0278; for Erbin, t(4) = 2.917, P = 0.0434; for ErbB4, t(4) = 3.421, P = 0.0267; for Nrg1, t(4) = 3.221, P = 0.0322; for Gng7, t(4) = 1.324, P = 0.256; for Npy, t(4) = 1.162, P = 0.31. e The messenger RNA expression level of Nrg1 but not ErbB4 was dramatically increased in the hippocampus. Student’s t test, for Nrg1, n = 6 mice per group, t(10) = 3.608, P = 0.0048; for ErbB4, n = 5 mice for CD group, n = 6 mice for KD group, t(9) = 0.9955, P = 0.3455. f Increased expression of Nrg1 and phosphorylated ErbB4 (P-ErbB4) proteins in the hippocampus of mice fed with K.D. Shown were representative blots of two mice from each group. GAPDH served as a loading control. g Quantitative analysis of the Western blot data in (f). Student’s t test, for NRG1, n = 5 CD-fed mice, n = 6 KD-fed mice, t(9) = 5.735, P = 0.0003; for ErbB4, n = 6 mice per group, t(10) = 1.384, P = 0.1965; for P-ErbB4, t(10) = 3.668, P = 0.0043

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