Fig. 4

KD treatment increases inhibitory synaptic activity in the hippocampus. a Diagram of hippocampal slice recording. Pyramidal neurons in the CA1 region were clamped in whole-cell configuration. Evoked postsynaptic currents were recorded under stimulation by using a concentric bipolar electrode. b Representative eIPSC traces. Scale bars, 100 ms and 1000 pA. c Increased amplitude of evoked inhibitory postsynaptic currents (eIPSCs) in the CA1 region of hippocampus in KD-fed mice. n = 17 neurons from 4 CD-fed mice; n = 15 neurons from 4 KD-fed mice. Two-way ANOVA, main effect, F_(1,267) = 11.9, P = 0.0007. d Representative spontaneous inhibitory postsynaptic current (sIPSC) traces. Scale bars, 2 s and 20 pA. e, f Increased sIPSC frequency but not amplitude in KD-fed mice. n = 18 neurons from 4 CD-fed mice; n = 15 neurons from 4 KD-fed mice. Student’s t test, for frequency, t₍₃₁₎ = 3.782, P = 0.0007; for amplitude, t₍₃₁₎ = 1.678, P = 0.1034. g Representative sweeps with inter-stimulus interval of pair-pulse stimulations at 100 ms. Scale bars, 50 ms and 100 pA. h Decreased PPRs in KD-fed mice. n = 17 neurons from 4 CD-fed mice; n = 15 neurons from 4 KD-fed mice. Two-way ANOVA, F_(1,90) = 5.871, P = 0.0174