Fig. 2

KD treatment suppresses epileptogenesis in KA-induced chronic seizure model. a Experimental design for spontaneous recurrent seizure (SRS) recording. SE was induced by the infusion of KA in the amygdala. One hour later, Seizure activity was terminated by injection of diazepam (i.p). Mice were then fed with CD or KD for 3 weeks, followed by 2 weeks of SRS recording. b reduced SRS number in mice fed with KD. n = 12 mice per group. Student’s t test, t₍₂₂₎ = 3.186, P = 0.0043. c Representative Timm staining images of brain sections from mice fed with CD or KD Magnified images of areas in dotted squares were shown on the bottom. Arrows, Timm granules in the supragranular region. Scale bar, 500 µm (top) and 200 µm (bottom). d Statistical analysis of the Timm index. n = 8 slices from 3 CD-fed mice; n = 9 slices from 3 KD-fed mice. Student’s t test, t₍₁₅₎ = 3.473, P = 0.0034