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Fig. 6 | Cell & Bioscience

Fig. 6

From: ACTA1 is inhibited by PAX3-FOXO1 through RhoA-MKL1-SRF signaling pathway and impairs cell proliferation, migration and tumor growth in Alveolar Rhabdomyosarcoma

Fig. 6

ACTA1 overexpression suppresses tumor growth originated from RH30 cells in nude mice. a Photograph of tumor growth in representative nude mice. Left flank: injected with RH30/vector cells. Right flank: injected with RH30/ACTA1 cells. Tumors in the left and right sides were indicated by red arrow and black arrow, respectively. b Growth curve of tumors originated from RH30/vector control and RH30/ACTA1 cell lines. Data are from two independent experiments. Error bars, SEM. c Western blot analyses for the detection of PAX3-FOXO1 and ACTA1 (Flag-ACTA1) protein expression in 3 representative tumor samples originated from RH30/vector (1,2,3) and RH30/ACTA1 (4,5,6) cell lines, respectively. Gapdh was used as a loading control. d H&E staining from representative tumor samples; e Flag staining for ACTA1 expression from representative tumor samples; f Staining for Ki67 expression from representative tumor samples. Arrow indicates ACTA1 (Flag-ACTA1) or Ki-67 expression. The expression level of Flag-ACTA1 or Ki-67 was judged with score 0 (negative), 1 (weak) and 2 (strong) based on the staining intensity and percentage of positive cells in the slide image

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