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Fig. 5 | Cell & Bioscience

Fig. 5

From: ACTA1 is inhibited by PAX3-FOXO1 through RhoA-MKL1-SRF signaling pathway and impairs cell proliferation, migration and tumor growth in Alveolar Rhabdomyosarcoma

Fig. 5

Effects of overexpressed ACTA1 on RH30 cell proliferation and migration. a Proliferation rate of stable cell lines RH30/vector and RH30/ACTA1 was determined by CCK-8 kit over 0-72 h (Lower panel) and assay of Flag-ACTA1expression in RH30/ACTA1 by western blot (Upper panel). b Wound healing assay was performed to detect the migration ability of the two cell lines RH30/vector and RH30/ACTA1. Upper panel: Representative images were taken at 0, 24 and 48 h. Lower panel: Relative migration rate of cells. *p < 0.05, compared with RH30/vector cells. c Transwell migration assay was performed in cell lines of RH30/vector and RH30/ACTA1, which showed that overexpressed ACTA1 could inhibit cell motility. *p < 0.05, compared with RH30/vector cells

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