Skip to main content
Fig. 1 | Cell & Bioscience

Fig. 1

From: ACTA1 is inhibited by PAX3-FOXO1 through RhoA-MKL1-SRF signaling pathway and impairs cell proliferation, migration and tumor growth in Alveolar Rhabdomyosarcoma

Fig. 1

Protein expression of ACTA1 gene in Alveolar Rhabdomyosarcoma (ARMS) cell lines. a Left: ACTA1 protein was examined by western blot in ARMS cell lines (RH30, RH4 and RH41). ACTA1 protein from mouse skeletal muscle tissue (m.sk) was shown as a control with same molecular weight as that from ARMS cells. α-tubulin is used as a loading control. Middle: Quantification of endogenous expression of PAX3-FOXO1 and ACTA1 proteins in cell lines normalized to α-tubulin and analyzed with ImageJ software. Right: Quantitative RT-PCR (qRT-PCR) analysis of ACTA1 mRNA expression in ARMS cell lines. b ACTA1 expression levels in normal vs primary sarcoma tumor samples from TCGA dataset (p = 1.25E-02). c Survival probability of sarcoma patients with low or high ACTA1 expression (modified from online UALCAN)

Back to article page

Cell & Bioscience

ISSN: 2045-3701

Contact us