Fig. 2

IL-15 expression increased in the placenta of GDM and in trophoblasts cultured with HG. a Fasting glucose in OGTT of participants. b Postprandial 1 h glucose (1 h) in OGTT of participants. c Postprandial 2 h glucose (2 h) in OGTT of participants. d IL-15 level in peripheral blood (PB) of patients. e RT-PCR detected expression of IL-15 mRNA in the placenta of participants. f, g Expression of IL-15 protein and representative images of western blot in the placenta of participants. h, i Representative Immunohistochemistry images and the relative optical density of IL-15 protein in the placenta of participants. Red arrows indicate placental villous. Bar = 25 μm. j The expression of IL-15 mRNA in trophoblast cell lines (HTR8/SVneo, JEG-3, JAR) in HG (25 mmol/L) as measured by RT-PCR. k, l The expression of IL-15 protein in trophoblast cell lines in HG detected by western blot and representative images of it. *P < 0.05, **P < 0.01, ***P < 0.001