Fig. 5

Integrin outside-in signaling induces Dab2-Ser24 phosphorylation. a, b Platelets with the indicated treatments were lysed for Western blotting analysis. The level of Dab2-Ser24 phosphorylation in the resting platelet lysate was arbitrarily set as 1. The data are presented as the mean ± SEM of 4 independent experiments. c Platelets with the indicated treatments were spread on 10-cm non-adhesive bacteriologic culture plates. The platelet lysates were collected for Western blotting analysis. Lysate from platelets treated with TPA (25 ng/ml) was used as a positive control. The proteolytic cleavage products of Dab2 were marked as *. d After spreading on coverslips, the platelet lysates were collected for Western blotting analysis. e, f Platelets were pre-incubated with the indicated concentrations of inhibitors then spread on coverslips. The platelet lysates were collected for Western blotting analysis. The proteolytic cleavage products of Dab2 were marked as *. The level of Dab2-Ser24 phosphorylation in platelets treated with DMSO and spread on Fg was arbitrarily set as 1. The data are presented as the mean ± SEM of 2–5 independent experiments. *p < 0.05; **p < 0.01