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Fig. 6 | Cell & Bioscience

Fig. 6

From: Neural stemness contributes to cell tumorigenicity

Fig. 6

Tissue differentiation in HCT 116 and U-118 MG xenograft tumors. a, b RT-qPCR detection of expression of genes for neuronal and neural stemness (a) and genes for mesodermal or/and endodermal tissue differentiation (b) in HCT 116 cells and tumors. c IHC detection of cell/tissue markers in HCT 116 tumor sections. Below the panels for marker expression are corresponding sections stained with HE. d, e RT-qPCR detection of expression of genes for neuronal and neural stemness (d) and genes for mesodermal or/and endodermal tissue differentiation (e) in U-118 MG cells and tumors. f IHC detection of cell/tissue markers in U-118 MG tumor sections. Below the panels for marker expression are corresponding sections stained with HE. In (a, b) and (d, e), significance of gene expression change was calculated based on experiments in triplicate using two-tailed Student’s t-test. Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. NS: not significant. In c and f, objective magnification for sections: 20×; insets: 40 × 

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