Fig. 1
Tumorigenicity and pluripotent differentiation potential of primNSCs. a Neural differentiation from mESCs cultured in serum-free medium, as shown by neurosphere formation after 6 days of culture. b Characterization of primNSC fate in neurospheres by IF detection of markers for pluripotency and neural stemness. Nuclei were counterstained with DAPI. c Tumors formed by injection of 0.5 × 106 of primNSCs. d Abundant diversity of tissue types in tumor. Displayed are a partial overview of tissue differentiation in a representative section and a few tissue types derived from ectoderm, endoderm and mesoderm. Original objective magnification: 4× for overview, 40× for specific tissues. e–g Comparison of expression of tissue or cell type-specific genes between primNSCs and tumor. Gene expression representing non-neural differentiation (e), neuronal differentiation (f) and neural stemness (g) was detected with RT-qPCR. Significance of gene expression change was calculated based on experiments in triplicate using two-tailed Student’s t-test. Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. NS: not significant