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Fig. 6 | Cell & Bioscience

Fig. 6

From: Probiotics mixture reinforces barrier function to ameliorate necrotizing enterocolitis by regulating PXR-JNK pathway

Fig. 6

PXR protected barrier function from LPS damage. a TEER changes in Caco-2 cell monolayers treated with LPS for 24 or 48 h. b and c Western blot analysis of claudin-1, occludin, and ZO-1 protein levels in Caco-2 cells treated with LPS (n = 4 per group, representative data from three independent experiments). d Western blot analysis of PXR in Caco-2 treated with LPS. e Confirmation of PXR overexpression by Western blot. f and g Western blot analysis of the components of tight junctions (Claudin-1, Occludin, and ZO-1) in Caco-2 cells treated with LPS for 48 h (n = 4 per group, representative data from three independent experiments). h FD4 flux of basolateral medium was measured after Caco-2 cells were treated with LPS or not treated for 48 h (n = 5 per group, representative data from four independent experiments). Data are expressed as mean values ± SD. Statistical analyses were performed with Student’s two-tailed unpaired t-test in (c and g), *p < 0.05; **p < 0.01; n.s., not significant; TEER, transepithelial electrical resistance; FD4, FITC-dextran 4 kDa

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