Fig. 6

miR-375 targeted RASD1 to activate the mTOR pathway. The target binding relationship between miR-375 and RASD1 was verified by dual-luciferase assay (a). Then RT-qPCR (b) and Western blot analysis (c) were used to detect the level of RASD1 in rat spinal cord and C8-DA1/SCI cells, and Western blot analysis was used to detect the levels of mTOR-related proteins in rat spinal cord and C8-DA1/SCI cells (d). Each experiment was repeated three times independently. The data are expressed by mean ± standard deviation. The data in panels B/C were analysed by one-way ANOVA, and data in panels a, d were analysed by two-way ANOVA, followed by Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01