Fig. 5

Mettl14-mediated m6A modification to promote the transformation from pri-miR-375 to miR-375. RT-qPCR was used to detect miR-375 and pri-miR-375 in cells after transfection for 24 h or spinal cord tissue of rats on the 30th day of each group (a, b), and then RT-qPCR was used to detect the effect of m6A mutation in pri-miR-375 on miR-375 processing (c); RIP assay was used to evaluate the effect of overexpression of Mettl14 on pri-miR-375 and miR-375 modified by m6A (d, e). Each experiment was repeated three times independently. The data are expressed by mean ± standard deviation. The data in panel B were analysed by the t test, and the data in panels a, c–e were analysed by one-way ANOVA, followed by Tukey’s multiple comparisons test. **p < 0.01